宠物犬布氏杆菌的PCR检测及部分序列分析

doi:10.11843/j.issn.0366-6964.2016.12.023

Abstract

Abstract:

Two cases of pet dogs were confirmed to be infected with Brucella canis based on detection to their excretion by use of the BRU real time PCR and conventional PCR kits, and the partial genetic sequences of the Brucella canis infected were characterized. The results showed that the Brucella strains from the pet dogs belong to the Brucella canis, and atypical canine brucellosis. Phylogenetic analysis of BSCP31 gene sequences showed 99%-100% nucleotide identity with the published sequences in GenBank, and OMP25 gene showed 99.9% nucleotide identity between Brucella canis xue1 strain and Brucella canis HSK A52141 strain. Sequence analysis on 16S rRNA showed the strains from French Bulldog and Teddy Dog belong to Brucella. These data might provide a valuble infomation for future screening and prevention of canine brucellosis.

CLC Number:

S852.612

SUN Ming，LIU Qiao-rong，SONG Wen-jing，QIAO Ming-ming，GU Qiang, ZHANG Qian，LIU Bo-hua，YANG Xue-song，CHEN Xi-zhao. PCR Detection and Partial Sequencing Analysis of Brucella canis from Pet Dogs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2520-2525.

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PCR Detection and Partial Sequencing Analysis of Brucella canis from Pet Dogs

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